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Oligo solutions were prepared in 10 mM Tris buffer, pH 7.
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This epitope was chosen because methylglyoxal is the principal AGE precursor induced in endothelial cells by hyperglycemia 18 , 19 , and the hydroimidazolone adduct is the major methylglyoxal-derived epitope
The demonstration in this report that inhibition of PARP completely blocks hyperglycemia-induced activation of both of these pathways suggests that PARP inhibitors might have unique clinical efficacy in preventing the development and progression of diabetic complications.
Description: In diabetic animal models, PARP inhibitors have been shown to improve myocardial dysfunction 39 and to prevent and reverse hyperglycemia-induced loss of endothelium-dependent vasodilatation 39 ,